The simulator is a PYMEAcquire configuration without any real hardware. It simulates a CCD camera which displays fake single molecule events generated from a given structure. It also simulates a number of other hardware components such as piezo translators and is intended as a testing/training platform for the acquisition of real data. It also allows different dye properties and behaviours to be invetigated. The simulator is launched by starting PYMEAcquire without specifying a startup file.